Pythons are disease vectors for multiple illness, including Salmonella, Chlamydia, Leptospirosis, Aeromoniasis, Campylobacteriosis, and Zygomycosis. These diseases may be transmitted to humans through excreted waste, open wounds, and contaminated water.

An introduced population of Burmese pythons (Python bivittatus) has existed as an invasive species in Florida's Everglades National Park since the late 1990s.

In Goblet of Fire and Order of the Phoenix, Nagini was likely portrayed as a Dumeril's Ground Boa, but in the last two films, she was portrayed as a reticulated python--the longest real-life snake species. Both instances contradict the book's description of her as a venomous snake.