In Show Biz Bugs (1957), Daffy arrives at a theater where he and Bugs Bunny are scheduled to appear. Furious that the rabbit's name on the marquee is above his in much larger letters, Daffy attempts to wow the audience with an act in which he drinks gasoline and swallows nitroglycerine, gunpowder, and uranium-238, jumps up and down to "shake well" and finally swallows a lit match that detonates the whole improbable mixture. When Bugs tells Daffy that the audience loves the act and wants more, Daffy, now a ghost floating upward, says that he can only do the act once.

In this 1946 cartoon, Daffy accidentally knocks himself out after reading the latest Dick Tracy comic book and dreams that he is the famous detective "Duck Tracy" investigating the theft of his own piggy bank.

In The Scarlet Pumpernickel (1950), Daffy has a middle name, Dumas as the writer of a swashbuckling script, a nod to Alexandre Dumas. In the Baby Looney Tunes episode "The Tattletale", Granny addresses Daffy as "Daffy Horatio Tiberius Duck". And in The Looney Tunes Show (2011), the middle names "Armando" and "Sheldon" are used.

In Tiny Toon Adventures, Daffy is a teacher at Acme Looniversity, where he is the hero and mentor of student Plucky Duck.

Daffy starred in 130 shorts in the golden age, making him the third-most frequent character in the Looney Tunes/Merrie Melodies cartoons, behind Bugs Bunny's 167 appearances and Porky Pig's 153 appearances.